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MaGIC and the Genomics Center present: Nanostring Spatial at any scale – morphology driven spatial transcriptomics and proteomics with the GeoMx Digital Spatial Profiler.
The GeoMx DSP is a novel digital technology (proprietary to NanoString) that is based on multiplexed measurement of protein and nucleic acids and offers unparalleled levels of precision coupled with the ability to quantify 150+ proteins and over 18,000 RNA targets on a formalin-fixed, paraffin-embedded (FFPE) or a fresh frozen (FF) tissue section on a microscope slide.
In contrast to the sequential analysis of multi-target immunohistochemistry (IHC) slides, the GeoMx DSP samples all protein and/or RNA analytes on a single slide. This not only shortens experiments and simplifies data analysis, but also provides a higher multiplexing capacity (18,000+ targets) all with spatial context from Formalin-Fixed, Paraffin-Embedded (FFPE) or fresh frozen (FF) tissue sections.
Based on NanoString’s proprietary digital barcoding technology, the GeoMx DSP platform measures local protein levels, and can be combined with RNA expression, within heterogeneous tissue samples. Combining both multiplexed nucleic acid and protein on the same platform gives researchers the ability to spatially resolve RNA when suitable antibodies do not exist. The GeoMx DSP assay is performed on the GeoMx DSP platform which includes imaging and fluidic components to capture spatial context, and current nCounter® or next-generation sequencing (NGS) instruments provide the quantification.

Oct 12, 2022 01:00 PM in Eastern Time (US and Canada)

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